KMID : 0043320100330040531
|
|
Archives of Pharmacal Research 2010 Volume.33 No. 4 p.531 ~ p.538
|
|
Syringin from Stem Bark of Fraxinus rhynchophylla Protects A¥â(25-35)- induced Toxicity in Neuronal Cells
|
|
Song Kyung-Sik
Yang Eun-Ju Kim Sang-In Ku Hyun-Yeong Lee Dong-Seok Lee Jong-Won Kim Yae-Sil Seong Yeon-Hee
|
|
Abstract
|
|
|
The medicinal herb Jinpi, derived from the dried stem barks of Fraxinus rhynchophylla belonging to Oleaceae is widely used as a variety of Korean folk remedies for anti-inflammatory, febricide, antidiarrhea, and antileukorrhea diseases. In the course of screening antidementia agents from natural products, F. rhynchophylla showed significant inhibitory activity toward A¥â(25-35)-induced neuronal cell death. An active principle was isolated and identified as syringin. When the neuroblastoma cells were exposed to 50 ¥ìM A¥â(25-35), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction rate (survival rate) decreased to 60.21 ¡¾ 2.16% over control while syringin treated ones recovered cell viability up to 79.12 ¡¾ 1.39% at 20 ¥ìM. In addition, 20 ¥ìM syringin almost completely removed A¥â(25-35)-induced reactive oxygen species. The neuroprotective effect of syringin seemed to be originated from the reduction of apoptosis since decrease in caspase-3 activity and expression, reduction in cleaved PARP, and DNA fragmentation were observed. These results suggest that F. rhynchophylla and syringin are expected to be useful for preventing A¥â(25-35)-induced neuronal cell damage.
|
|
KEYWORD
|
|
Alzheimer¡¯s disease, Fraxinus rhynchphylla, Syringin, ¥â-Amyloid fibrils, Apoptosis
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|